Effect of GSH on NO peak heights obtained with a high concentration of DEA/NO. Shown are pre- and post-Cu2+ peak heights for 100 µM DEA/NO in the absence and presence of 1 mM GSH and 5 mM MgCl2. Experimental conditions: 100 µM DEA/NO, 1 mM GSH as indicated, 4 mM CuSO4 as indicated, 1000 U/ml SOD, 0.1 mM DTPA, 5 mM MgCl2 as indicated, and 50 mM TEA (pH 7.4) in 0.5 ml at 37 °C.