Table 1.

Effects of various thiols on the pre- and post-Cu²⁺ NO peak.

Thiol	NO peak height (µM)
	Before Cu2+	After Cu2+
−	0.69±0.02	n.d.
GSH	0.42±0.04	0.69±0.04
GSSG	0.74±0.01	n.d.
Cys	0.45±0.03	n.d.
DTT	0.51±0.05	0.065±0.001
NAC	0.43±0.08	0.49±0.09
β-ME	0.38±0.02	0.51±0.01

NO peak heights from 1 µM DEA/NO before and after addition of 4 mM CuSO₄ were measured in the absence (−) or presence of various thiols at 1 mM: glutathione (GSH), oxidized glutathione (GSSG), cysteine (Cys), dithiothreitol (DTT), N-acetylcysteine (NAC), or β-mercaptoethanol (β-ME). Experimental conditions: 1 µM DEA/NO, 1 mM thiol as indicated, 4 mM CuSO₄, 1000 U/ml SOD, 0.1 mM DTPA, 5 mM MgCl₂, and 50 mM TEA (pH 7.4) in 0.5 ml at 37 °C. n.d., not detectable.