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. 2013 Aug 6;7:121. doi: 10.3389/fncel.2013.00121

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Copyright © 2013 Westerholz, de Lima and Voigt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A–D) Network activity histograms of representative fields recorded at 13–14 DIV with Fluo-4 calcium imaging. Bars show the percentage of active neurons against time. (E) The graph shows the network burst frequency for T3⁻ (gray bars) and T3⁺ (black bars) in control sets of cultures (Ctr), and in cultures treated chronically with K252a or rapamycin (Rap). (F–K) Graphs show the cumulative probability for attendance (% of active cells participating in single activity events) (F–H) and single cell frequency (I–K) for each of control (F,I) and drug-treated culture sets (K252a: G,J; rapamycin: H,K).