Table 5.
T3− | T3+ | T3−× T3+ | Drug × T3 | |||||
---|---|---|---|---|---|---|---|---|
Mean ± s.e.m. | n | P# | Mean ± s.e.m. | n | P# | P* | P& | |
L-GABA (cells/mm2) | ||||||||
Control | 4.4 ± 0.4 | 60 | 4.4 ± 0.3 | 60 | 0.937 | |||
BDNF | 4.7 ± 0.4 | 60 | 0.643 | 5 ± 1 | 50 | 0.253 | 0.138 | 0.255 |
K252a | 5.1 ± 0.5 | 60 | 0.603 | 5.1 ± 0.5 | 60 | 0.338 | 0.739 | 0.806 |
Rapamycin | 5.6 ± 0.4 | 60 | 0.070 | 4.4 ± 0.5 | 50 | 0.803 | 0.078 | 0.163 |
0.310 | 0.308 | |||||||
non-GABA (cells/mm2) | ||||||||
Control | 708 ± 46 | 60 | 521 ± 29 | 60 | 0.003 | |||
BDNF | 569 ± 38 | 60 | 0.029 | 465 ± 36 | 50 | 0.129 | 0.074 | 0.543 |
K252a | 763 ± 53 | 60 | 0.605 | 553 ± 31 | 60 | 0.537 | 0.004 | 0.939 |
Rapamycin | 967 ± 72 | 60 | 0.007 | 618 ± 51 | 50 | 0.326 | <0.001 | 0.398 |
<0.001 | 0.095 | |||||||
GABA SOMA SIZE (μm2) | ||||||||
Control | 308 ± 7 | 120 | 351 ± 8 | 120 | <0.001 | |||
BDNF | 308 ± 6 | 120 | 0.835 | 348 ± 6 | 120 | 0.572 | <0.001 | 0.825 |
K252a | 246 ± 4 | 120 | <0.001 | 264 ± 5 | 120 | <0.001 | 0.004 | 0.300 |
Rapamycin | 187 ± 4 | 120 | <0.001 | 241 ± 4 | 120 | <0.001 | <0.001 | <0.001 |
<0.001 | <0.001 | |||||||
GABA AXON LENGTH (μm/field) | ||||||||
Control | 951 ± 40 | 60 | 715 ± 42 | 60 | <0.001 | |||
BDNF | 994 ± 42 | 60 | 0.657 | 762 ± 47 | 60 | 0.487 | 0.001 | 0.703 |
K252a | 1020 ± 50 | 60 | 0.300 | 943 ± 38 | 60 | <0.001 | 0.397 | 0.027 |
Rapamycin | 676 ± 28 | 60 | <0.001 | 624 ± 44 | 60 | 0.160 | 0.312 | 0.009 |
<0.001 | <0.001 | |||||||
GABA BOUTONS (counts/field) | ||||||||
Control | 47 ± 3 | 60 | 64 ± 5 | 60 | 0.009 | |||
BDNF | 67 ± 3 | 60 | <0.001 | 74 ± 6 | 60 | 0.309 | 0.836 | 0.038 |
K252a | 58 ± 3 | 60 | 0.009 | 83 ± 3 | 60 | <0.001 | <0.001 | 0.174 |
Rapamycin | 58 ± 3 | 60 | 0.003 | 76 ± 6 | 60 | 0.282 | 0.200 | 0.244 |
<0.001 | 0.023 | |||||||
MW-RST | MW-RST | 2w-ANOVA | ||||||
ANOVA | ANOVA |
Statistics as in Table 4. n, number of fields analyzed per condition.