FIGURE 5.

Expression of VSFP3.1TagRFP from the Ubi-10 promoter and targeting to nuclear membrane in root cells. Images show expression of the intensity-based VSFP3.1TagRFP from the Ubi-10 promoter at the plasma membrane (A) and the nuclear membrane (B, +WPP) in cells of the root maturation zone. In (B), only some background fluorescence is seen in the plasma membrane. The four faint spots to the right in (B) are root hair nuclei. The excitation and emission wavelengths of TagRFP are 545 nm and 605 nm, respectively. Images shown are fluorescence wide field images acquired after focusing on the top cell layers of the root or the root hair.