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. 2013 Aug 6;4:223. doi: 10.3389/fimmu.2013.00223

Figure 4.

Figure 4

A positive correlation is observed between CD8αα expression and the size of the CD8α+CD8βlow population. (A) Representative FACS data showing TL-tetramer binding to CD3+ and CD3+CD4+ T cells of peripheral blood lymphocytes. CD8αβ binding by TL-tetramer is blocked by α-CD8β antibody clone 2ST8.5H7. Control samples shown on histogram on right (Negative controls: unstained and TL + α − CD8α; Positive control; TL). Data shown from a patient with chronic HBV. Data representative of 11 repeat experiments. (B) Representative FACS data using TL-tetramer staining to demonstrate CD8αα expression by CD3+CD161++/CD161+/CD161− T cells in HCs. Data representative of three repeat experiments. (C) Representative FACS data of TL-tetramer staining/CD8αα expression by CD3+CD161++ and CD3+CD161++Vα7.2+ T cell subsets in a HC. Data representative to three repeat experiments. (D) Comparison of TL-tetramer expression/CD8αα expression between CD161++/CD161+/CD161−T cell subsets in HCs and patients with chronic HBV, HCV, and HIV-1 infections. (*p < 0.05, One-way ANOVAs). (E) Correlative analysis between the proportion of CD3+CD161− TL-tetramer positive cells and the % of CD161−CD8βlow cells as a proportion of the CD161−CD8α+ population in patients with chronic HBV, HCV, and HIV-1 infections, r = 0.7541, p = 0.0118, Pearson.