Table 1.
Quantitation of lipid content of various CHO compartments
| PC (% of PL) | SM (% of PL) | CHOL (mol/mol) | Protein/Lipid (wt/wt) | |
|---|---|---|---|---|
| CHOGIC | 46 | 38 | 0.96 | 0.13 |
| CHO total-DRM | 35 | 28 | 0.71 | 0.17 |
| CHO Golgi | 37 | 14 | 0.57a | 1.6 |
| CHO cells | 49bc | 8bc | 0.35a | 8.2d |
| CHO PM | 39c | 18c | 0.59e | Not determined |
PC, phosphatidylcholine; PM, plasma membrane. Lipids were measured and quantified as described in MATERIALS AND METHODS. Various CHO compartments were isolated as described in MATERIALS AND METHODS. As indicated, some data were published recently by Brügger et al. (1997)
b
and by Sandhoff et al. (1999).
a
The lipid content of CHO-derived plasma membranes was determined by Cezanne et al. (1992)
c
and Warnock et al. (1993).
d
From CHO postnuclear supernatant. PL, total phospholipid; mol/mol, mol cholesterol/mol total phospholipid. The data are expressed as mean values (±10%) from two different determinations.