Figure 3.

Effects of triptolide and anti-rheumatic drugs on DNA fragmentation of RSF. Cells were incubated with triptolide (closed diamond), bucillamine (closed triangle), methotrexate (closed circle), or D-penicillamine (open triangle) for 24 h, after which fragmented DNA in the cytoplasm was measured by enzyme immunoassay and the fold induction of DNA fragmentation was calculated relative to the control value (untreated cells). Data are the mean ± S.D. for triplicate cultures and representative results from 5 independent experiments are shown.