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Morphological changes of RSF (a, b) and detection of apoptosis by the TUNEL assay (c, d). Cells were incubated for 24 h without (a, c) or with (b, d) triptolide at 100 nM. Brown cells are apoptotic cells that show TUNEL staining, while blue cells are normal cells stained with methylgreen. Original magnification × 200.
