Figure 5.

Effect of triptolide on caspase activity in RSF. RSF were cultured without agents for 24 h, or were incubated with 100 nM triptolide or 100 nM triptolide plus 10 μM Z-VAD-FMK, and then caspase activity was measured by the CaspACE™ assay system (Promega) as the absorbance at 405 nm. Data are the mean ± S.D. for 3 independent experiments. *, p < 0.01 versus untreated control cells and versus cells treated with triptolide plus Z-VAD-FMK. Significance was evaluated by Student's t-test with a Bonferroni's correction.