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Conditions that protect against relocation of nuclear proteins. Fpr3p was localized 0 and 10 min after hypertonic shock in YPH500 cells carrying either the control plasmid pRS314 or a high-copy plasmid (pPS1739) for expression of Hog1p. In parallel experiments, YPH500 or hsf1–1 (MYY260) cells were incubated for 1 h at 37°C before being returned to 23°C and subjected to hypertonic shock for 0 or 10 min.
