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. Author manuscript; available in PMC: 2013 Aug 6.
Published in final edited form as: Stem Cells. 2011 Mar;29(3):505–516. doi: 10.1002/stem.598

Figure 2.

Figure 2

Developmental potential of myoblast-derived induced pluripotent stem cells (iPSCs). (A): Sections of teratoma formation after subcutaneous injection of myoblast-derived iPSCs into Nod:Scid mice. H&E staining shows iPSC contribution to all three germ layers, keratinized epithelium (ectoderm), cuboidal structures (endoderm), and skeletal muscle (mesoderm). Scale bar = 50 µm. (B): Myoblast-derived iPSCs generated from Oct4-GFP mice efficiently incorporated into GFP+ inner cell mass (arrows) of blastocysts after aggregation with CD1 mouse embryos. Non-aggregated blastocysts show auto fluorescence background (arrowheads). Scale bar = 25 µm. (C): Myoblast-derived iPSCs generated from Oct4-GFP mice contributed to the germ line cells (arrows), which expressed Oct4-GFP in the gonad of 13.5 dpc chimeric embryos. Control nonchimeric gonad shows no GFP staining. Scale bar = 100 µm. (D): X-gal staining for chimeric embryo (12.5 dpc) after aggregation with myoblastderived iPSCs generated from Myf5-Cre:R26R mice (left panel). Chimeric mice generated from Myf5-Cre:R26R myoblast-derived iPSCs showed mixed coat color at 3 weeks age (middle panel). The mice indicate medium contributed chimera (mixed color), CD1 nursing mother (white color), and high-contributed chimera (agouti color). The agouti color pups indicate germ line transmission of Myf5-Cre:R26R myoblast-derived iPSCs (right panel). (E): Genomic polymerase chain reaction (PCR) confirmed the chimerism of fetal mice generated from Oct4-GFP myoblastderived iPSCs. The chimeric fetal tissues contained both wild-type (434 bp) and Oct4-GFP transgene (234 bp) alleles: Sk, Br, Li, Ht, Ts, Ey, Ri, To, TA, Tt. PCR results for control DNAs prepared from tail tips of wild-type (þ/þ), homozygous (tg/tg), and heterozygous (+/tg) Oct4-GFP mice are also shown. Abbreviations: Br, brain; Ey, eye; GFP, green fluorescent protein; Ht, heart; Li, liver; Ri, rib; Sk, skin; TA, tibialis anterior muscle; Ts, testis; To, tongue muscle; Tt, tail tip. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]