Figure 4. Direct targets of N1ICD/RBPJ include transcription factors of the WNT, SHH, and Hippo pathway.
(A) Venn diagram summarizing the overlap among ChIP-seq and transcriptome analyses to identify 98 genes with highest confidence of being direct targets of N1ICD/RBPJ complex. (B) Gene set analysis 73 of most enriched pathways represented by the 98 target genes. (C) RT-PCR validation of selected genes in wildtype and N1ICD;GFAP-Cre cortices at E15.5 (student t-test, 0.0001<p< 0.04). (D) Endogenous NOTCH1 target gene expression in control vs. RBPJ−/− NSCs, measured by realtime RT-PCR. (n=7, p< 0.05 for all except Tbr2, p<0.17) (E) Realtime RT-PCR analysis of C17-2 cells transiently transfected with N1ICD (CaN1) or EGFP control expression vector (p<0.04, n=3). Also see Supplementary Figure 3.