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. 2013 Apr 15;12(8):2048–2059. doi: 10.1074/mcp.M112.026716

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 3. — A, SILAC ratios for the H3 K79 peptide EIAQDF⁷⁹K(Me_0–3)TDLR across all 44 yeast mutants. B, SILAC ratios for the H3 K56 peptide FQ⁵⁶K(Ace_0–1)STELLIR across all 44 yeast mutants. WT1 is from plasmid pMP3 that carries both wild type histone H3 and H4 genes (HHT2-HHF2). WT2 is from plasmid pQQ18 that carries both wild type histone H2A and H2B. To compare the peptide level between mutants, raw H/L ratios of each peptide are normalized using the filtered robust linear regression normalization factor. Error bars correspond to two-fold of the sample based standard deviation (see Experimental Procedures and Supplemental data for description and example).