Fig. 2.

Depth of the detected phosphoproteomes. The number of identifications was similar between different phospho-motif antibody enrichment methods ranging from 617 to 827 phosphopeptides after filtering out duplicates. Around 67% of the mapped phosphosites were novel in reference to the PhosphoSitePlus database. Overall, 68% of the identified peptides were singly phosphorylated, whereas 32% of the peptides carried multiple phosphorylation sites.