Fig. 5.

HS-dependent growth factor signaling identified in phosphoproteomics analysis and verification by p-RTK array assay. A, HS-dependent growth factor signaling that was identified by IPA analysis. B, Mouse p-RTK array analysis. There are 39 different receptor tyrosine kinases on the array including HGF receptor (HGFR), IGF-1R, and Mer as indicated. The RTK array layout was provided as supplemental Fig. S4. C, Compared with Ext1f/f MLECs, the phosphorylation levels of most of the receptor tyrosine kinases, including HGFR and IGF-1R, but not Mer, EphA2, and EphA7, are lower in the Ext1−/− cells. The data shown are representative of three repeated experiments.