Table I. List of the identified protein. For GAPDH and RihC in table are reported the results obtained from spot 8 and 10 respectively. Number of peptides, Peptide sequences and Individual ion scores are reported in supplementary Table S1.
Spot | Protein | NCBI ID | MW (Da) | pI | Score | Seq. cov. (%) | Peptide sequence | Obs. mass | Theo. mass | Δm (ppm) |
---|---|---|---|---|---|---|---|---|---|---|
1 | Phosphoketolase | gi 148544892 | 91346 | 5.09 | 2888 | 39 | NQEUINLFVTSK | 750,8326 | 750,8334 | 1,0655 |
2 | Pyruvate kinase | gi 148543982 | 51793 | 5.06 | 4077 | 72 | ELVUHVLNHGVLGS | 869,4182 | 869,4185 | 0,3451 |
UNELGKPVITATQMLDSMQENPRPTR | 759,3545 | 759,3552 | 0,9218 | |||||||
3 | Arginine deiminase | gi 148543680 | 46214 | 5.39 | 1803 | 73 | UMoxSCPIVR | 543,7015 | 543,7023 | 1,4714 |
UMSCPIVR | 535,7056 | 535,7049 | −1,3067 | |||||||
DQQAUIGDGITINHMTFK | 699,6362 | 699,6373 | 1,5722 | |||||||
4 | 6-Phosphogluconate dehydrogenase | gi 184154309 | 53498 | 5.56 | 2005 | 56 | AGUIIR | 369,1643 | 369,1640 | −0,8126 |
AEEDGKPUVAYIGPNGAGHYVK | 794,0203 | 794,0206 | 0,3778 | |||||||
5,7,8 | Glyceraldehyde 3-phosphate dehydrogenase | gi 184153036 | 35971 | 5.41 | 2913 | 71 | DDIIVSAGSUTTSCLAPMAK | 1072,9598 | 1072,9589 | −0,8388 |
NDGVDFVLEUTGFYTSAEK | 1100,4534 | 1100,4528 | −0,5452 | |||||||
6 | Ornithine carbamoyltransferase | gi 148543661 | 37536 | 5.21 | 1791 | 58 | USFEVGAK | 473,1824 | 473,1826 | 0,4227 |
USFEVGAKDEGAHVTYLGPSGSHIGHK | 963,4326 | 963,4337 | 1,1417 | |||||||
ATENPNVLFEHULPAFHNLDTEVGK | 725,8341 | 725,8334 | −0,9644 | |||||||
9,10 | Ribonucleoside hydrolase RihC | gi 148543341 | 32552 | 4.70 | 1211 | 60 | VULDIDAEYFNK | 767,8191 | 767,8200 | 1,1721 |