Fig. 3.
Immunostaining of in vivo biotinylated tissues. Various biotinylated tissues were sectioned for staining with streptavidin: A, liver; B, intestine; C, kidney; D, CAM; E, granulation tissue; F, xenografted tumor (U87). Biotinylated proteins (red) were detected in basement membranes of the different tissues. Images were taken with a Zeiss confocal microscope. Scale bars: B, 10 μm; A, C, D, 25 μm; E, 50 μm. A grid was used in the wound experiment (blue autofluorescence) (E). Nuclei were stained with DAPI (blue).