Table II. List of proteins from the tumor/CAM sample that were unambiguously identified as human.
| Gene | Number of peptides | Products |
|---|---|---|
| Extracellular, secreted, or membranar proteins | ||
| ANXA5 | 3 | Annexin A5 |
| FN1 | 3 | Fibronectin 1 |
| TGFBI | 3 | Transforming growth factor, beta-induced, 68 kDa |
| TNC | 3 | Tenascin C |
| ANXA1 | 2 | Annexin A1 |
| ANXA2 | 2 | Annexin A2 |
| COL1A1 | 2 | Collagen, type I, alpha 1 |
| ITGA5 | 2 | Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) |
| COL4A1 | 1 | Collagen, type IV, alpha 1 |
| LOXL1 | 1 | Lysyl oxidase-like 1 |
| PDIA4 | 1 | Protein disulfide isomerase family A, member 4 |
| PDIA6 | 1 | Protein disulfide isomerase family A, member 6 |
| PRDX4 | 1 | Peroxiredoxin 4 |
| SBSN | 1 | Suprabasin |
| Glycolytic or fermentation enzyme | ||
| PCCA | 9 | Propionyl CoA carboxylase, alpha polypeptide |
| GAPDH | 7 | Glyceraldehyde-3-phosphate dehydrogenase |
| PKM2 | 7 | Pyruvate kinase, muscle |
| PC | 5 | Pyruvate carboxylase |
| ENO1 | 4 | Enolase 1 (alpha) |
| ALDOA | 3 | Aldolase A, fructose-bisphosphate |
| LDHA | 3 | Lactate dehydrogenase A |
| MCCC1 | 3 | Methylcrotonoyl-CoA carboxylase 1 (alpha) |
| TPI1 | 2 | Triosephosphate isomerase 1 |
| ALDOC | 1 | Aldolase C, fructose-bisphosphate |
| ENO2 | 1 | Enolase 2 (gamma, neuronal) |
| GPI | 1 | Glucose-6-phosphate isomerase |
| LDHAL6B | 1 | Lactate dehydrogenase A-like 6B |
| PGK1 | 1 | Phosphoglycerate kinase 1 |
| Cytoskeleton proteins | ||
| VIM | 7 | Vimentin |
| LMNA | 1 | Lamin A/C |
| MYH14 | 1 | Myosin, heavy chain 14, non-muscle |
| MYH9 | 1 | Myosin, heavy chain 9, non-muscle |
| TUBB8 | 1 | Tubulin, beta 8 |
| Stress proteins | ||
| AKR1B1 | 2 | Aldo-keto reductase family 1, member B1 (aldose reductase) |
| HSPD1 | 2 | Heat shock 60-kDa protein 1 (chaperonin) |
| HSP90AB1 | 1 | Heat shock protein 90-kDa alpha (cytosolic), class B member 1 |
| HSPA5 | 1 | Heat shock 70-kDa protein 5 (glucose-regulated protein, 78 kDa) |
| PRDX4 | 1 | Peroxiredoxin 4 |
| Others | ||
| PHEX | 1 | Phosphate regulating endopeptidase homolog, X-linked |
| AEBP1 | 1 | AE binding protein 1 |
| EEF2 | 1 | Eukaryotic translation elongation factor 2 |
Note: This table lists glioma genes identified by peptide matching. Genes had peptides specific to human (glioma cells) with eight genes matching at least seven different human specific peptides. Ten genes had promiscuous but human-specific peptides (data not shown).