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. 2013 May 14;12(8):2313–2323. doi: 10.1074/mcp.M112.023853

Fig. 5.

Fig. 5.

CS-0777-P phosphatase activity of recombinant proteins. A, Specific CS-0777-P phosphatase activity of the recombinant proteins. Candidate proteins (ALPL, ALPI, ALPP, and ALPPL) and the negative control (EGFP) were expressed in the mammalian cell line, and the enzyme activity was tested. The recombinant proteins were quantified by Western blotting. The data are shown as the average of duplicates with error bars indicating the minimum and maximum values (n = 2). B, Specific CS-0777-P phosphatase activity of the recombinant proteins. Candidate proteins (LPP1, LPP2, LPP3, SPP1, and SPP2) and the negative control (EGFP) were expressed in the mammalian cell line, and the enzyme activity was tested. The recombinant proteins were quantified by GFP fluorescence. The data are shown as the average of duplicates with error bars representing the minimum and maximum values (n = 2).