Table I. Purification table of CS-0777-P phosphatase from the human kidney.
Step | Protein conc. [μg/ml] | Activity [U/ml] | Volume [ml] | Total protein [mg] | Total activity [U] | Specific content [U/mg] | Step fold change | Overall fold change | Step recovery [%] | Overall recovery [%] |
---|---|---|---|---|---|---|---|---|---|---|
Human kidney homogenate | 16543 | 57 | 10 | 165 | 570 | 3.4 | - | 1 | - | 100 |
1) Centrifugation | 4339 | 176 | 10 | 43 | 1760 | 41 | 11.8 | 12 | 309 | 309 |
2) Multimodal anion exchange (Capto adhere) | 188 | 18 | 5 | 0.9 | 90 | 96 | 2.4 | 28 | 5.1 | 16 |
3) Anion Exchange (Mono Q) | 101 | 42 | 1 | 0.1 | 42 | 416 | 4.3 | 121 | 47 | 7.4 |
4) Gel filtration (Superdex 200) | 10 | 7 | 0.05 | 0.0005 | 0.4 | 711 | 1.7 | 206 | 0.8 | 0.1 |