Skip to main content
. 2013 May 14;12(8):2313–2323. doi: 10.1074/mcp.M112.023853

Table I. Purification table of CS-0777-P phosphatase from the human kidney.

Step Protein conc. [μg/ml] Activity [U/ml] Volume [ml] Total protein [mg] Total activity [U] Specific content [U/mg] Step fold change Overall fold change Step recovery [%] Overall recovery [%]
Human kidney homogenate 16543 57 10 165 570 3.4 - 1 - 100
1) Centrifugation 4339 176 10 43 1760 41 11.8 12 309 309
2) Multimodal anion exchange (Capto adhere) 188 18 5 0.9 90 96 2.4 28 5.1 16
3) Anion Exchange (Mono Q) 101 42 1 0.1 42 416 4.3 121 47 7.4
4) Gel filtration (Superdex 200) 10 7 0.05 0.0005 0.4 711 1.7 206 0.8 0.1