Figure 2.

Rab11a interaction with truncated myosin Vb tail constructs. The structure of the myosin Vb tail is shown schematically including a region of coiled-coil structure and the terminal globular region. Amino acid numbers in parentheses correspond to the sequence of murine myosin Vb (myr 6), whereas the nonbracketed numbers refer to the amino acid sequence of the rabbit myosin Vb tail clone shown in Figure 1. Truncations of the myosin Vb tail sequence were assembled in pADGAL and cotransfected into yeast with either pBDGAL-Rab11a or pBDGAL-Rab11aS20V. Lifts of yeast colonies were then exposed to Xgal and development of blue color was interpreted as a positive interaction (+). Results with Rab11aS20V were identical to those with Rab11a wild type.