Figure 4.

Localization of endogenous myosin Vb in MDCK cells stably expressing GFP-Rab11a. GFP-Rab11a/MDCK cells were grown at confluence for 4 d on permeable filters. Cells were fixed after no treatment (a–c), treatment with nocodazole (d–f), or treatment with taxol (g–i). Cells were triple imaged for GFP-Rab11a (a, d, and g), endogenous myosin Vb immunostaining (b, e, and h), and ZO-1 immunostaining (c, f, and i). Each panel represents X-Y confocal projection reconstructions. Arrowheads indicate the position used for X-Z reconstructions shown in the bottom portion of each panel. The results are representative of two separate experiments. Bar, 1 μm (a–c), 2 μm (d–i).