Figure 6.

Effects of overexpression of GFP-myosin Vb tail and GFP-myosin Va tail on transferrin trafficking in HeLa cells. To assess the effects on transferrin trafficking GFP-myosin Vb tail (A) or GFP-myosin Va tail (B) were transiently transfected into HeLa cells. Twenty-four hours after transfection, cells were serum starved for 30 min and then allowed to endocytose Cy3-transferrin for 60 min. After uptake, some cells were allowed to recover in 10% fetal bovine serum-containing medium for 2 h. Cells were imaged for GFP, Cy3-transferrin, and immunostaining for endogenous transferrin receptor. The results are representative of four separate experiments. Bar, 5 μm.