Figure 8.

GFP-myosin Vb tail alters apical recycling system morphology in MDCK cells. MDCK were transiently transfected with GFP-myosin Vb tail and grown for 3 d at confluence on permeable filters. (a–f) Cells were then exposed to either no treatment (a–c) or treatment with nocodazole (d–f). Fixed cells were triple imaged for GFP (a and d), endogenous Rab11a (b and e), and ZO-1 (c and f). (g–i) GFP-myosin Vb tail transfected MDCK cells were fixed and triple imaged for GFP (g), the Golgi marker p58 (h), and ZO-1 (i). The results are representative of three separate experiments. Bar, 5 μm.