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. 2013 Feb 13;43(4):889–896. doi: 10.1002/eji.201243008

Figure 1.

Figure 1

AMPKα1null T cells activate, proliferate, and function normally. (A) Immunoblot analysis of total AMPKα1 and GSK3 in control and AMPKα1null CD4 thymocytes, two experiments. (B) FSC, CD69, CD25, CD71, CD98, and CD44 expression by control (gray shade) and AMPKα1null (thick line) OT1 LN cells activated in vitro for 24 h with SIINFEKL compared with IL-7 (thin line). (C) IL-2 maintained proliferation in vitro, control (filled squares) and AMPKα1null (open circles) of OT1 cytotoxic T lymphocytes (CTLs), average ± SD, three experiments. (D) IFN-γ secretion (pg/million CTLs) 3 h SIINFEKL restimulation of control and AMPKα1null OT1 CTLs. Data are shown as mean + SEM of n = 3 mice/genotype representing three experiments. (E) CFSE profile, CD62L, CD44, and FSC analysis of control (top panel) and AMPKα1null (bottom panel) OT1 cells adoptively cotransferred into Ly5.1 recipient mice, 2 days after immunization with LPS + SIINFEKL (open) or LPS (gray shade), two experiments, two to three recipients.