Figure 5.

AMPK is dispensable for CD8 cells during primary infection but essential during secondary infection. (A) Frequency OT1 cells among total CD8 cells, average ± SD, eight Ly5.1 recipients, control (filled squares), and AMPKα1^null (open circles) in blood day 7, 10, 14, and 35 after recombinant Listeria monocytogenes OVA (rLMOVA) infection. (B) IL-7Rα and KLRG1 expression by OT1 cells in the blood, control (upper panel) and AMPKα1^null (lower panel) day 7, 14, and 39 after primary rLMOVA infection. Data shown are representative from two experiments. (C) Frequency of cotransferred control and AMPKα1^null TCR transgenic OT1 cells day 6 post secondary challenge in the spleen, liver, and bone marrow. Each symbol represents CD45 congenically marked OT1 cells among total CD8 cells in individual recipients. Data shown are representative from two experiments, (n = 4–6 recipients), paired t-test. (D) Frequency (left panel) and absolute number (right panel) of MHC class H-2K^b+SIINFEKL pentamer+ cells among total CD8 cells in the spleen. Data are shown as mean ± SD (n = 3–5 mice/genotype/time-point). Control AMPKα1^fl/fl mice (black) and AMPKα1^null CD4creAMPKα1^fl/fl (open) mice days 7, 14, and 28 after primary rLMOVA infection. (E) Frequency of pentamer+ cells among CD8 cells day 6 post secondary rLMOVA challenge in control AMPKα1^fl/fl mice (black square) compared with AMPKα1^null CD4creAMPKα1^fl/fl mice (open circle) in spleen, liver, and bone marrow. Each symbol represents one mouse, Mann–Whitney test.