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. 2004 Feb 6;101(10):3338–3345. doi: 10.1073/pnas.0307875101

Fig. 3.

Fig. 3.

Heart defects in Fbw7-/- embryos. (A–D) A wild-type embryo at ED9.5 illustrating normal heart morphogenesis. (A) Left-lateral view shows chamber formation of the future left ventricle (LV) and left atrium (LA) following the inflow tract (IFT). (B) Ventral view of wild-type heart illustrating the differentiation of the future right ventricle (RV) and LV along the linear heart tube. (C) Right-lateral view shows the RV and the appearance of a mature outflow tract (OFT). (D) Cranial view of the mature hearts displays normal organization of the presumptive chambers (LV and RV) with respect to IFT and OFT. (E–H) Fbw7-/- hearts at ED9.5 illustrate defective development. (E) Left-lateral view shows delayed chamber formation. IFT leads directly to primitive LV structure, indicated by white arrowhead. (F) Ventral view shows that the linear heart develops only a primitive LV, with no apparent RV development. (G) Right-lateral view depicts absence of RV chamber formation. (H) Cranial view of mutant heart. (I and J) Left-lateral view of Nkx2.5 in situ hybridization on embryos at ED9.5. (I) Expression pattern of Nkx2.5 in all cells of the developing heart tube. (J) Reduced expression of Nkx2.5 at ventral-most portion of the heart curvature, the future left ventricle of the Fbw7-/- heart (white arrowhead). (K and L) Embryos at ED10.5. (K) Wild-type heart with well differentiated chambers. (L) Fbw7-/- heart remains a linear heart tube with very little chamber formation. BA-1, first branchial arch.