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. 2013 Jun 4;21(8):1579–1591. doi: 10.1038/mt.2013.78

Figure 3.

Figure 3

Transfected HEK293 cells secrete Sema3C or Sema3A in vivo. (a) Diagram showing the transplantation sites for HEK293 cells (blue circles) and the location of subsequent panels. (b) In animals that received Sema3C-transfected grafts, HEK293 cells were identified with the antihuman nuclei (HuNu) antibody, and Sema3C immunoreactivity was detected 1 week after grafting. (c–f) Detection of (c,d) Sema3A and (e,f) Sema3C in the (c,e) injection path, and close to the substantia nigra (SN), where (d,f) grafted TH-positive cells were present 1 week after transplantation. The non-transplanted contralateral hemispheres of these rats did not show any labeling with Sema 3A nor 3C antibodies (data not shown), and Sema3C was absent in rats grafted with mock-transfected HEK293 cells (Supplementary Figure S1). Nuclei were marked with Hoechst 33258. Scale bar in b applies to b–f = 50 µm.