Figure 3.
Comparative transduction levels from canine adenovirus type 2 (CAV-2) and lentivirus in adult rat brain. (A) Rostro-caudal gene transduction (grey) after single injection of 3 × 109 virus particle (vp) CAVGFP (n=4; left) and double injection of 2 × 107 vp lenti-GFP (n=4; right) determined from the median animal in each virus group. Black dots represent cortical injection sites. The staining pattern of green fluorescent protein (GFP) expression was transcribed onto stereotaxic atlas plates.27 (B) Representative cortical images of GFP epifluorescence from lentivirus or CAV-2 transduction 7 days after stereotactic injection; scale bar, 100 μm (top) and 20 μm (bottom). Images taken from the primary somatosensory cortex (S1FL) at coronal level Bregma −0.12 mm (indicated with an X on atlas plate image). (C) Volume of tissue transduced by each viral vector quantified from area under the curve analysis across seven coronal levels. Data presented as mean±s.e.m.; *P<0.0002 versus lentivirus using Student's unpaired t-test.