Fig. 2.
Cooverexpression of Bcl-XL and dominant-inhibitory TRAIL receptors delays luminal clearance in MCF-10A acini. (a) Indicated cell lines were cultured in Matrigel for the indicated number of days (d). Images are representative confocal crossections through the center of acini immunostained with laminin 5 (red) and Ki67 (green). Nuclei were counterstained with TO-PRO III (blue). (Scale bars, 25 μm.) (b) The percentage of acini with two or more intact nuclei located within the lumen was quantified. Numbers are means of three independent experiments performed with a minimum of 100 acini scored for each cell line at all time points. *, P = 0.0005, by Fisher's exact test with Monte Carlo analysis.