Figure 5.

CUL1 siRNA inhibits the gelatinolytic activities of pro-MMP-9 and increased the expression of TIMP-1 and TIMP-2. (a) HTR8/SVneo cells transfected with indicated siRNAs were subjected to total proteins extraction and western blotting to detect expression of CUL1 (also in c). Serum-free culture medium was collected for gelatin zymography assay. (b) Statistical analysis of the zymographic results in (a) (t-test; **P<0.01). (c) Serum-free culture medium (for the detection of TIMP-1 and -2) or the whole cell proteins (for the detection of CUL1) of HTR8/SVneo cells transfected with indicated siRNAs were used for western blotting using indicated antibodies