Figure 3.
Acetate increases levels of TUNEL-positive cells. Apoptosis analysis in HCT-15 and RKO cells by TUNEL assay, after incubation with IC50 and 2 × IC50 acetate concentrations (70 mM and 140 mM for HCT-15; 110 mM and 220 mM for RKO) for 48 h. (a) Analysis of TUNEL assay in HCT15 and RKO cells. Values represent mean±S.E.M. of at least three independent experiments. *P≤0.05; ***P≤0.001 compared with negative control cells. (b) Representative images ( × 200) of DAPI (4',6-diamidino-2-phenylindole), FITC (fluorescein isothiocyanate) and merged were obtained by fluorescence microscopy. PC, phase contrast