Figure 5.

ZnCl₂ restored p53 wild-type conformation and SY5Y-APP cell sensitivity against oxidative insult. Cells were treated with 100 μℳ ZnCl₂ for 24 h and then processed for p53 conformational analysis and cell viability. (a and b) ELISA immunoassay carried out with PAb240 (unfolded p53) (a) and PAb1620 (wild type p53) (b) antibodies. (c and d) After 24 h ZnCl₂ pretreatment, mock (c) and SY5Y-APP (d) cells were exposed to H₂O₂ pulse (5 min) in the presence or absence of the p53 inhibitor, Pif, followed by incubation with fresh medium for additional 24 h. Cell viability was evaluated by MTT assay. Data were expressed as % of cell survival versus respective controls. Values were the mean±S.E.M. of three different experiments. *P<0.001 ZnCl₂ followed by H₂O₂-treated SY5Y-APP cells versus ZnCl₂ pre-treated SY5Y-APP cells