Fig. 6.

Presence of basonuclin 2 mRNA in various tissues. (A) A Northern blot containing 2 μg of poly(A)⁺ RNA prepared from various human tissues was purchased from Clontech. RNAs were successively hybridized to a 733-bp basonuclin 2-specific probe (Left), an 802-bp basonuclin 1 probe (Right), and a human actin probe. Testis, uterus, and intestine gave appreciable signals for basonuclin 2, prostate gave a weak signal, and colon gave a trace signal. All other tissues were negative. The human basonuclin 2 mRNA was estimated at 6 kb. Testis was the only tissue that gave a signal for basonuclin 1; two transcripts at 4.6 and 3.2 kb were present, as described in ref. 3. The sources of the RNAs are indicated. (B) RT-PCR analysis of total RNA prepared from various adult mouse tissues by using oligonucleotide primers designed to amplify specifically an 894-bp basonuclin 2 fragment and an 810-bp basonuclin 1 fragment. The PCR products were resolved on a 1% agarose gel and visualized by ethidium bromide staining. The mouse basonuclin 2 mRNA is found in all tissues examined. It is particularly abundant in testis and kidney and less abundant in epidermis and small intestine. Liver and colon contain trace amounts (the image is overexposed). The basonuclin 1 mRNA is only found in testis and epidermis, in which it is less abundant than the basonuclin 2 mRNA. (C) RT-PCR analysis of total RNA prepared from human cultured keratinocytes. The mRNA for basonuclin 1 (802-bp fragment) is ≈10 times more abundant than that for basonuclin 2 (733-bp fragment).