Figure 1.

Deletion of GGA2 in combination with gga1Δ or chc1-ts affects α-factor maturation. chc1-ts (GPY982; lane 1), chc1-ts gga2Δ (GPY2273; lane 2), chc1-ts gga1Δ (GPY2275; lane 3), gga2Δ (GPY2149; lane 4), gga1Δ (GPY2151; lane 5), and gga1Δ gga2Δ (GPY2385; lane 6) cells were metabolically labeled at 24°C for 45 min, and secreted α-factor was immunoprecipitated from the culture supernatants. Samples were analyzed by SDS-PAGE and fluorography. Precursor levels of α-factor were quantified by phosphoimage analysis.