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. 2001 Jun;12(6):1885–1896. doi: 10.1091/mbc.12.6.1885

Figure 2.

Figure 2

Deletion of GGA2 in combination with gga1Δ or any AP β subunit gene deletion does not affect ALP transport to the vacuole. (A) Wild-type (WT, SEY6210; lanes 1–5), gga2Δ (GPY2149; lanes 6–10), gga1Δ gga2Δ (GPY2385; lanes 11–15) cells. (B) apl2Δ gga2Δ (GPY2420–3C; 1–5), apl1Δ gga2Δ (GPY2422–9D; lanes 6–10), and apl6Δ gga2Δ (GPY2425–29C; lanes 11–15) cells were metabolically labeled for 10 min at 30°C and then subjected to a chase for the indicated times. ALP was immunoprecipitated from cell lysates and analyzed by SDS-PAGE and fluorography. Precursor (proALP) and mature (mALP) forms are indicated.