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Deletion of the AP-1 β subunit gene in combination with gga2Δ strongly affects α-factor maturation. Wild-type (WT, SEY6210; lane 1), apl2Δ gga2Δ (GPY2420–3C; lane 2), apl1Δ gga2Δ (GPY2422–9D; lane 3), apl6Δ gga2Δ (GPY2425–29C; lane 4), and apl4Δ gga2Δ (GPY2426–38C; lane 5) cells were processed and quantified as described in the legend to Figure 1.
