Figure 4.

Transport of CPS to the vacuole is impaired in gga1Δ gga2Δ and gga2Δ apl2Δ cells. Wild-type (WT, SEY6210; lanes 1–3), gga2Δ (GPY2149; lanes 4–6), gga1Δ gga2Δ (GPY2385; lanes 7–9), apl2Δ gga2Δ (GPY2420–3C; lanes 10–12), apl1Δ gga2Δ (GPY2422–9D; lanes 13–15), and apl6Δ gga2Δ (GPY2425–29C; lanes 16–18) cells were metabolically labeled for 10 min at 30°C and subjected to a chase for the indicated time points. Lysates were prepared and CPS was immunoprecipitated and treated with endoglycosidase H before SDS-PAGE and fluorography. Precursor (proCPS) and the mature forms (mCPS) are indicated.