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Gga2p cofractionates with clathrin-coated vesicles. Shown is an S-1000 column chromatography elution profile of a high-speed pellet fractionation (60 min, 100,000 × g) from Gga2p-HA–expressing cells (GPY2373). Fractions were analyzed by SDS-PAGE and immunoblotting with antibodies against Chc1p and HA.
