Fig. 1.

Isolation and identification of CpG1, an ENU-induced point mutation in TLR9. (A) Trace file of amplified genomic DNA from homozygous mutant mice (upper chromatogram) and normal animals (lower chromatogram). (Left) Location of the mutation within the 11th LRR in the TLR9 ectodomain. (Lower) Multiple alignment of all human and mouse TLR protein sequences reveals that the mutation resides in a region shared only by TLRs 7, 8, and 9. At left, the identity of each sequence is indicated. H, human paralog; M, mouse paralog. Note that TLRs 11 and 12 are nearest homologs, and TLR13 is most closely related to TLR3. (B) Transfection-based assay of TLR9 signaling activity. Error bars indicate the SD of duplicate transfections.