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Impairment of NK and NKT cell activation after MCMV inoculation in TLR9 and MyD88 mutant mice. Purified splenic cells from uninfected or MCMV-infected animals were recovered 36 h postinoculation and gated against NK1.1 and TCRβ surface markers. (Left) IFN-γ intracellular staining (and isotype antibody as control) for NK (NK1.1+TCRβ-). (Right) Results for NKT cells (NK1.1+TCRβ+). Mean values ± SD are indicated (n = 4 mice).
