Table 3. Analysis of contacts, surface area, and SC differences between D44.1-HEL and F10.6.6-HEL.
| ΔASA total
|
ΔASA polar
|
ΔASA no polar
|
No. of contacts
|
SC
|
Average contact distances
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment | D44 | F10 | D44 | F10 | D44 | F10 | D44 | F10 | D44 | F10 | D44 | F10 |
| HEL | 607.4 | 633.9 | 303.4 | 321.4 | 304.0 | 312.5 | ||||||
| Fv | 617.4 | 676.0 | 306.3 | 313.4 | 311.1 | 362.6 | 93 | 129 | −143.67 | −162.08 | ||
| VH | 393.6 | 413.8 | 175.2 | 178.5 | 218.4 | 235.3 | 57 | 78 | −67.18 | −69.01 | ||
| H1 | 183.9 | 189.6 | 78.0 | 83.5 | 105.9 | 106.1 | 30 | 36 | 3.56 | 3.44 | ||
| H2 | 128.4 | 136.2 | 65.1 | 64.0 | 63.3 | 72.2 | 11 | 18 | 3.46 | 3.49 | ||
| FR3 | 28.8 | 28.9 | 0.9 | 1.9 | 27.9 | 27.0 | 8 | 11 | 3.65 | 3.70 | ||
| H3 | 52.4 | 59.1 | 31.2 | 29.1 | 21.3 | 30.0 | 8 | 13 | 3.68 | 3.46 | ||
| VL | 223.8 | 262.2 | 131.1 | 134.9 | 92.7 | 127.3 | 36 | 51 | −61.57 | −73.57 | ||
| L1 | 33.5 | 64.1 | 31.9 | 48.7 | 1.6 | 15.4 | 1 | 1 | 3.44 | 3.76 | ||
| L2 | 27.3 | 31.2 | 14.4 | 15.6 | 12.9 | 15.6 | 1 | 6 | 3.63 | 3.74 | ||
| L3 | 163.0 | 166.9 | 84.8 | 70.6 | 78.2 | 96.3 | 34 | 44 | 3.66 | 3.39 | ||
Average contact distances were calculated for both crystallographic complexes of each antibody by using a cutoff of 4.0 Å.