Fig. 6.

In vitro polyadenylation reactions reveal differences in specificities of inhibition between sera from the A3 and A6 immunized animals. In vitro polyadenylation reactions were performed by using SV40 late mRNA as substrate RNA (SVL) and HeLa nuclear extract, in the absence (lane 1) or presence (lanes 2-8) of specific antibodies. Lane 1, Polyadenylation reaction with no antibodies; lane 8, polyadenylation reaction in the presence of negative control antibody (anti-myc tag antibody 9E10). The A3 immunized rabbit sera (lanes 2-4) exhibit no inhibition of polyadenylation until late in disease course when anti-A6 antibodies begin to appear. The A6 immunized animal sera (lanes 5-7) exhibit strong inhibition of polyadenylation early in disease, which tapers off as anti-A6 antibody titers decrease over time.