Relationship between sperm content (per U G6PDH activity) of L-carnitine (A, C) and L-glutamate (B, D) of the control sample (Protocol c: abscissa) and the relative effectiveness of Protocol A over Protocol B (A/B ratio of sperm numbers, ordinate) in maintaining sperm migration into surrogate mucus at the 3 cm mark (A, B) and the 1 cm mark (C, D) after addition and removal of ethylene glycol (EG: A, B) and propane-1, 2-diol (PD2: C, D).