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. 2009 Aug 17;11(5):557–565. doi: 10.1038/aja.2009.21

Figure 3.

Figure 3

In vitro growth and morphology of human spermatogonial stem cells (SSCs). (A) – (F): Human SSCs were cultured on human embryonic stem cell-derived fibroblast-like cells (hdFs) with the addition of Glial cell line-derived neurotrophic fator (GDNF). Note that human SSCs form compact colonies. Human SSCs at 1 day in vitro (A), 3 days (B), 5 days (C), 10 days (D), 1 month (E) and 2 months (F). (G): Human SSCs cultured under feeder-free conditions on day 3. Note that human SSCs cannot form compact colonies without hdF feeder layer support. (H): Human SSCs cultured on hdFs without the addition of GDNF on day 3. Note that human SSC colonies cannot be maintained in the absence of GDNF. Bars = 50 μm.