Table 1. Polymerase chain reaction (PCR) primers used for the amplification of reference gene candidates used in this study.
| Gene | Genebank accession | Forward primer (5′–3′) | Reverse primer (5′–3′) | Size (bp) |
|---|---|---|---|---|
| OCT4 | NM_002701.4 | CTGGGTTGATCCTCGGACCT | CCATCGGAGTTGCTCTCCA | 243 |
| SOX3 | NM_005634.2 | CGCGGGTTCCTGCTGATTT | CGGGGTTCTTGAGTTCAGTCT | 222 |
| STRA8 | NM_182489.1 | CTGTGGCAGAACCTCTCGG | GAACCTCACTTTTGTCCAGGAA | 238 |
| NGN3 | NM_020999.2 | ACTGTCCAAGTGACCCGTGA | TCAGTGCCAACTCGCTCTTAG | 205 |
| DAZL | NM_001351.2 | ACACTGAAACTTATATGCAGCC | CGGAGGTACAACATAGCTCCTT | 182 |
| NOTCH1 | NM_017617.3 | CGCTGACGGAGTACAAGTG | GGTAGGAGCCGACCTCGTT | 246 |
| C-KIT | NM_000222.2 | GTTCTGCTCCTACTGCTTCGC | TAACAGCCTAATCTCGTCGCC | 135 |
| β-actin | NM_001101.2 | CATGTACGTTGCTATCCAGGC | CTCCTTAATGTCACGCACGAT | 250 |