Fig. 7.
(a) RT-PCR along rat dissected nephron segment. The PCR product amplified was electrophoresed and stained with ethidium bromide (Upper). Subsequently, gels were transferred and hybridized with [32P]dCTP-labeled rat Oatp4c1 cDNA (Lower). Glm, glomerulus; PCT, proximal convoluted tubule; PST, proximal straight tubule; MAL, medullary thick ascending limb; CAL, cortical thick ascending limb; CCD, cortical collecting duct; OMCD, outer medullary collecting duct; IMCD, inner medullary collecting duct. (b) Immunohistochemistry of rat Oatp4c1 in rat kidney. The rat Oatp4c1 immunoreactivity was significantly detected at the basolateral membrane of the proximal tubule cell. (Scale bar = 100 μm.)