Application
|
Isolation buffer
|
Destination buffer
|
RNA extraction (e.g. for amplification and transcriptomics) |
1x First-Strand buffer (Invitrogen), 1 mM DTT, 4% RNAseOUT |
RNA extraction buffer |
DNA extraction (e.g. for bisulfite sequencing) |
100 mM Tris, 10 mM EDTA, pH 8 (TE) |
DNA extraction buffer or 1x TE buffer |
GFP reporter analysis |
1 M Glycin in 0.5x MS1
|
1 M Glycin in 0.5x MS |
GUS reporter analysis |
staining buffer2 without X-Gluc (substrate) |
staining buffer with X-Gluc (substrate) |
FISH & Immunostaining |
100 mM Phosphate Buffered Saline (PBS) |
activated Acryl:Bisacryl mix (33:3) on a Superfrost Plus slide -polymerize for 30 min |