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. 2013 Jun 7;(76):50371. doi: 10.3791/50371
Application Isolation buffer Destination buffer
RNA extraction (e.g. for amplification and transcriptomics) 1x First-Strand buffer (Invitrogen), 1 mM DTT, 4% RNAseOUT RNA extraction buffer
DNA extraction (e.g. for bisulfite sequencing) 100 mM Tris, 10 mM EDTA, pH 8 (TE) DNA extraction buffer or 1x TE buffer
GFP reporter analysis 1 M Glycin in 0.5x MS1 1 M Glycin in 0.5x MS
GUS reporter analysis staining buffer2 without X-Gluc (substrate) staining buffer with X-Gluc (substrate)
FISH & Immunostaining 100 mM Phosphate Buffered Saline (PBS) activated Acryl:Bisacryl mix (33:3) on a Superfrost Plus slide -polymerize for 30 min