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Type III secretion by WT CR and its 41 LEE mutants. (A) General protein secretion profile of CR and its mutants. (B) Tir and EspB secretion analyzed by Western blot with anti-Tir and anti-EspB sera. (C) Secretion profile of ΔsepL, Δrorf6, ΔescN (TTS mutant), and their double mutants. Secreted proteins were concentrated from supernatants of bacterial cultures grown in DMEM and analyzed by 12% SDS/PAGE and Coomassie blue G250 staining (A and C) or Western blot (B).
